Test Code #


CPT Code(s) #

86038, 86039 (if reflexed), 86255

Test Name

Antinuclear Antibodies (ANA) Titer/Pattern on HEp-2

If Profile, Includes Tests:


Disease Name:

LE, other Connective Tissue Diseases

Type of Study:

Serum Studies


Indirect Immunofluorescence



Reference Range:

Negative: <1:40


Titer & ANA pattern reported on all positives.


Assay performed daily Monday-Friday. Report availability is within 48 hours from the time of specimen receipt.

Specimen Requirements:

Collect 5-10 ml of blood in a red top or serum separator tube. If possible, separate serum from clot and place into red capped tube provided with Beutner Laboratories collection kits. If separation facilities are not available, the blood can be sent in the tube used for collection.

Sample Stability:

Stable at ambient temperature during shipment. If sample is stored prior to shipment, it is stable refrigerated (2-8ºC) up to five days and frozen (-20ºC or lower) up to one year.

Clinical Relevance:

This test is for detection of antibodies to nuclear antigens. The homogeneous pattern is primarily associated with Systemic Lupus Erythematosus (SLE). Antibodies to centromere antigens are highly specific for Calcinosis, Raynaud's phenomenon, Esophageal dysmotility, Sclerodactyly, and Telangiectasia, commonly referred to as CREST syndrome, a limited form of systemic sclerosis (SSc). Centromere antibodies can also occasionally be found in low titers in some other autoimmune variants. Antinuclear antibodies with a speckled pattern are commonly associated with SLE, although they do occur in some cases of Sj�gren's syndrome and mixed connective tissue disorders. ANA antibodies with a nucleolar pattern are commonly associated with SSc, although they do occur in some cases of SLE and overlap syndromes. Overlap syndromes include dermatomyositis/polymyositis.

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