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Test #

Split Skin IgG & IgG4

CPT Code(s) #

88346, 88350

If Profile, Includes Tests:


Disease Name:

Pemphigoid, Epidermolysis Bullosa Acquisita (EBA), Bullous Pemphigoid, Bullous LE, Laminin 332 Autoimmunity

Type of Study:

Serum Studies


Indirect Immunofluorescence


Split Human Skin

Reference Range:

Negative: <1:5




Assay performed daily Monday-Friday. Report availability is within 48 hours from the time of specimen receipt

Specimen Requirements:

Collect 5-10 ml of blood in a red top or serum separator tube. If possible, separate serum from clot and place into red capped tube provided with Beutner Laboratories collection kits. If separation facilities are not available, the blood can be sent in the tube used for collection.

Sample Stability:

Stable at ambient temperature during shipment. If sample is stored prior to shipment, it is stable refrigerated (2-8ºC) up to five days and frozen (-20ºC or lower) up to one year.

Clinical Relevance:

Epidermolysis bullosa acquisita (EBA) can mimic bullous pemphigoid (BP) clinically, histologically and immunologically. In this indirect immunofluorescence assay, EBA and BP antibodies can be distinguished by their localization in skin that is split at the lamina lucida. This test and added follow up tests help differentiate EBA/bullous LE/anti-epiligrin cicatricial pemphigoid from bullous pemphigoid.

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