009
Test #
Envoplakin Antibody
CPT Code(s) #
83520
If Profile, Includes Tests:
N/A
Disease Name:
Paraneoplastic Pemphigus
Type of Study:
Serum Studies
Methodology:
ELISA
Substrate:
N/A
Reference Range:
Ratio <1.0:Negative
Ratio ≥1.0:Positive
Units:
Ratio
Schedule:
Assay performed once weekly. Report availability is within one week from time of specimen receipt.
Specimen Requirements:
Collect 2-5 ml of blood in a red top Vacutainer or serum separator (SST) tube, allow blood to clot, and centrifuge. Label tube appropriately.
Sample Stability:
Room temperature = stable during shipment; 2ºC to 8ºC = 5 days; -25ºC to -15ºC = 1 year
Clinical Relevance:
Paraneoplastic pemphigus (PNP) is a life-threatening form of pemphigus that is associated with a neoplasm (e.g., non-Hodgkin's lymphoma, chronic lymphocytic leukemia, Castleman tumor, thymoma, sarcoma, Waldenstrom's macroglobulinemia). Pathogenesis is based on a combination of humoral and cellular autoimmune responses. Circulating autoantibodies are directed against multiple antigens, including predominantly plakins (envoplakin, periplakin, desmoplakin I, desmoplakin II, epiplakin), plectin, Dsg1, Dsg3 and BP230. Due to their high specificity (91-100%), anti-envoplakin autoantibodies are considered an important diagnostic marker for paraneoplastic pemphigus. This ELISA is % sensitive in detecting antibodies to envoplakin in PNP patients.
