009
Test #
Envoplakin Antibodies - IgG (ELISA)
CPT Code(s) #
83520
If Profile, Includes Tests:
N/A
Disease Name:
paraneoplastic pemphigus (PNP)/paraneoplastic autoimmune multiorgan syndrome (PAMS)
Type of Study:
Serum
Methodology:
ELISA
Substrate:
N/A
Reference Range:
Negative: Ratio < 1.0
Positive: Ratio ≥ 1.0
Units:
Ratio
Schedule:
Assay performed once weekly. Report availability is within 7 days from time of specimen receipt.
Specimen Requirements:
Serum: Serum Separator Tube or red top tube, 2-5 mL preferred, 0.5 mL minimum sample volume. Separate serum from cells ASAP or within 2 hours of collection.
Sample Stability:
Storage & Stability:
Room temperature = 3 days
2-8ºC = 7 days
-15ºC to -25ºC = samples in long-term storage
Clinical Relevance:
Paraneoplastic pemphigus (PNP) is a life-threatening form of pemphigus that is associated with a neoplasm (e.g., non-Hodgkin's lymphoma, chronic lymphocytic leukemia, Castleman tumor, thymoma, sarcoma, Waldenstrom's macroglobulinemia). Pathogenesis is based on a combination of humoral and cellular autoimmune responses. Circulating autoantibodies are directed against multiple antigens, including predominantly plakins (envoplakin, periplakin, desmoplakin I, desmoplakin II, epiplakin), plectin, Dsg1, Dsg3 and BP230. Due to their high specificity (91-100%), anti-envoplakin autoantibodies are considered an important diagnostic marker for paraneoplastic pemphigus. This ELISA is % sensitive in detecting antibodies to envoplakin in PNP patients.
