022
Test #
Epidermal Transglutaminase Antibodies - IgA (eTG ELISA)
CPT Code(s) #
83520
If Profile, Includes Tests:
N/A
Disease Name:
Dermatitis Herpetiformis
Type of Study:
Serum
Methodology:
ELISA
Substrate:
N/A
Reference Range:
Negative: < 16 AU/mL
Intermediate: 16-22 AU/mL
Positive: ≥ 22 AU/mL
Units:
AU/mL
Schedule:
Assay performed once every two weeks. Report availability is within 14 days from the time of specimen receipt.
Specimen Requirements:
Serum: Serum Separator Tube or red top tube, 2-5 mL preferred, 0.5 mL minimum sample volume. Separate serum from cells
Sample Stability:
Storage & Stability:
Room temperature = 3 days
2-8ºC = 7 days
-15ºC to -25ºC = samples in long-term storage
Clinical Relevance:
Patients with Dermatitis Herpetiformis (DH) have gluten induced circulating IgA autoantibodies to tissue transglutaminase (TG2) and epidermal transglutaminase (TG3). Recent data indicated that DH is IgA-TG3 immunocomplex mediated disease developing in some gluten sensitive enteropathy (GSE) patients. There is pathogenic TG3 deposition in the papillary dermis and small blood vessels in skin of patients, colocalized with granular IgA deposits. IgA epidermal transglutaminase antibodies ELISA is a sensitive test for initial diagnosis and follow up of patients with DH.
