013a
Test #
Pemphigus/Pemphigoid Antibody Titer IgG, IgG1 & IgG4*
CPT Code(s) #
88346, 88350 x2
If Profile, Includes Tests:
Disease Name:
Pemphigus, paraneoplastic pemphigus (PNP)/paraneoplastic autoimmune multiorgan syndrome (PAMS), Pemphigoid, Bullous Pemphigoid, EBA, Bullous Lupus Erythematosus, Bullous Diseases
Type of Study:
Serum
Methodology:
Indirect Immunofluorescence
Substrate:
Primate Esophagus
Reference Range:
Negative: < 1:10
Positive: ≥ 1:10
Units:
Titer
Schedule:
Assay performed daily Monday-Friday. Report availability is within 48-72 hours from the time of specimen receipt.
Specimen Requirements:
Collect 2-5 ml of blood in a red top Vacutainer or serum separator (SST) tube, allow blood to clot, and centrifuge. Label tube appropriately.
Sample Stability:
Room temperature = stable during shipment
2ºC to 8ºC = 5 days
-25ºC to -15ºC = 1 year
Clinical Relevance:
The detection of anti-skin (IC and BMZ) antibodies aids in the diagnosis, and sometimes prognosis, of autoimmune bullous diseases including pemphigus, pemphigoid, cicatricial pemphigoid, and epidermolysis bullosa acquisita (EBA). Epithelial intercellular antibodies are diagnostic for pemphigus and occur in over 90% of patients with active forms. Antibodies to basement membrane antigens of stratified squamous epithelium occur in about 70% of active bullous pemphigoid, 50% of EBA and 10% of cicatricial pemphigoid patients.
